Identification of antigen-specific TCR sequences based on biological and statistical enrichment in unselected individuals.

ABSTRACT: Recent advances in high-throughput T cell receptor (TCR) sequencing have allowed for new insights into the human TCR repertoire. However, methods for capturing antigen-specific repertoires remain an area of development. Here, we describe a potentially novel approach that utilizes both a biological and statistical enrichment to define putatively antigen-specific complementarity-determining region 3 (CDR3) repertoires in unselected individuals. The biological enrichment entailed FACS of in vitro antigen-activated memory CD4+ T cells, followed by TCRβ sequencing. The resulting TCRβ sequences were then filtered by selecting those that are statistically enriched when compared with their frequency in the autologous resting T cell compartment. Applying this method to define putatively peanut prot

SUBMITTER: Smith NP 

PROVIDER: S-EPMC8410028 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature