RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.

ABSTRACT: RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome. This assay measures activity of the hepatitis E virus (HEV) RdRp. In contrast to protocols available to assay the RdRp activity of many other viruses, this assay utilizes DIG-11-UTP as a nonradioactive alternative to ³²P-UTP, thereby increasing the convenience of performing the assay.

SUBMITTER: Nair VP 

PROVIDER: S-EPMC8410430 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature