Propofol Suppresses Microglia Inflammation by Targeting TGM2/NF-κB Signaling.

ABSTRACT:

Background

Propofol is a known intravenous hypnotic drug used for induction and maintenance of sedation and general anesthesia. Emerging studies also reveal a neuroprotective effect of propofol in diverse diseases of neuronal injuries via modulating microglia activation. In this study, we aimed to uncover the downstream targets of propofol in this process.

Methods

RNA sequencing analysis to identify genes implicated in the propofol-mediated neuroprotective effect. Quantitative real-time PCR, enzyme-linked immunosorbent assay, and Western blotting analysis were performed to analyze inflammatory gene expression, cytokine levels, and TGM2. BV2 cells and primary microglia were used for functional verification and mechanism studies.

Results

The multifunctional enzyme trans

SUBMITTER: Hou Y 

PROVIDER: S-EPMC8410446 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature