AC092127.1-miR-451a-AE binding protein 2 Signaling Facilitates Malignant Properties of Breast Cancer.

ABSTRACT:

Purpose

The purpose of the current study was to explore the functions and potential mechanism of miR-451a in breast cancer (BC).

Methods

Quantitative reverse transcription real-time polymerase chain reaction was used to analyze the expression of miR-451a in human normal mammary cells (MCF-10A) and BC cells. Colony formation assay, terminal-deoxynucleoitidyl transferase mediated nick end labeling assay and transwell assays were conducted to validate the effect of miR-451a on proliferation, apoptosis, migration and invasion of BC cells, respectively. RNA pull-down, RNA immunoprecipitation and luciferase reporter assays were applied to investigate the upstream and downstream mechanisms of miR-451a in BC cells.

Results

MiR-451a was expressed at a low level in BC cells. Ov

SUBMITTER: Zhang X 

PROVIDER: S-EPMC8410618 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature