DebarcodeR increases fluorescent cell barcoding capacity and accuracy.

ABSTRACT: Fluorescent cell barcoding (FCB) enables efficient collection of tens to hundreds of flow cytometry samples by covalently marking cells with varying concentration of spectrally distinct dyes. A key consideration in FCB is to balance the density of dye barcodes, the complexity of cells in the sample, and the desired accuracy of the debarcoding. Unfortunately, barcoding bench and computational methods have not benefited from the high dimensional revolution in cytometry due to a lack of automated computational tools that effectively balance these common cytometry needs. DebarcodeR addresses these unmet needs by providing a framework for computational debarcoding augmented by improvements to experimental methods. Adaptive regression modeling accounted for differential dye uptake between differ

SUBMITTER: Reisman BJ 

PROVIDER: S-EPMC8410645 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature