Distinct nuclear compartment-associated genome architecture in the developing mammalian brain.

ABSTRACT: Nuclear compartments are thought to play a role in three-dimensional genome organization and gene expression. In mammalian brain, the architecture and dynamics of nuclear compartment-associated genome organization is not known. In this study, we developed Genome Organization using CUT and RUN Technology (GO-CaRT) to map genomic interactions with two nuclear compartments-the nuclear lamina and nuclear speckles-from different regions of the developing mouse, macaque and human brain. Lamina-associated domain (LAD) architecture in cells in vivo is distinct from that of cultured cells, including major differences in LADs previously considered to be cell type invariant. In the mouse and human forebrain, dorsal and ventral neural precursor cells have differences in LAD architecture that correspon

SUBMITTER: Ahanger SH 

PROVIDER: S-EPMC8410652 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature