A plant host, Nicotiana benthamiana, enables the production and study of fungal lignin-degrading enzymes.

ABSTRACT: Lignin has significant potential as an abundant and renewable source for commodity chemicals yet remains vastly underutilized. Efforts towards engineering a biochemical route to the valorization of lignin are currently limited by the lack of a suitable heterologous host for the production of lignin-degrading enzymes. Here, we show that expression of fungal genes in Nicotiana benthamiana enables production of members from seven major classes of enzymes associated with lignin degradation (23 of 35 tested) in soluble form for direct use in lignin activity assays. We combinatorially characterized a subset of these enzymes in the context of model lignin dimer oxidation, revealing that fine-tuned coupling of peroxide-generators to peroxidases results in more extensive C-C bond cleavage compared

SUBMITTER: Khlystov NA 

PROVIDER: S-EPMC8410833 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature