Rational design of hyperstable antibacterial peptides for food preservation.

ABSTRACT: We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic assays are used to rationally develop a set of peptides. pH and thermostability assays were performed to demonstrate robust antimicrobial activity post-treatment with high temperatures and at wide pH ranges. We have also investigated the mode of action of these hyperstable peptides using membrane permeability assays, electron microscopy, and molecular dynamics simulations. Notably, through mutational studies, we show that these peptides elicit their antibacterial action via both membrane destabilization an

SUBMITTER: Vishweshwaraiah YL 

PROVIDER: S-EPMC8410836 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature