Delayed KCNQ1/KCNE1 assembly on the cell surface helps I_Ks fulfil its function as a repolarization reserve in the heart.

ABSTRACT:

Key points

In adult ventricular myocytes, the slow delayed rectifier (I_Ks ) channels are distributed on the surface sarcolemma, not t-tubules. In adult ventricular myocytes, KCNQ1 and KCNE1 have distinct cell surface and cytoplasmic pools. KCNQ1 and KCNE1 traffic from the endoplasmic reticulum to the plasma membrane by separate routes, and assemble into I_Ks channels on the cell surface. Liquid chromatography/tandem mass spectrometry applied to affinity-purified KCNQ1 and KCNE1 interacting proteins reveals novel interactors involved in protein trafficking and assembly. Microtubule plus-end binding protein 1 (EB1) binds KCNQ1 preferentially in its dimer form, and promotes KCNQ1 to reach the cell surface. An LQT1-associated mutation, Y111C, reduces KCNQ1 binding

SUBMITTER: Wilson ZT 

PROVIDER: S-EPMC8411300 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature