Transcriptome-wide m⁶A methylome during osteogenic differentiation of human adipose-derived stem cells.

ABSTRACT:

Objectives

Adipose-derived stem cells are frequently used for bone regeneration both in vitro and in vivo. N⁶-methyladenosine (m⁶A) is the most abundant post-transcriptional modification on eukaryotic RNAs and plays multifaceted roles in development and diseases. However, the regulatory mechanisms of m⁶A in osteogenic differentiation of human adipose-derived stem cells (hASCs) remain elusive. The present study aimed to build the transcriptome-wide m⁶A methylome during the osteogenic differentiation of hASCs.

Materials and methods

hASCs were harvested after being cultured in a basic or osteogenic medium for 7 days, and the osteogenic differentiation was validated by alkaline phosphatase (ALP) and Alizarin Red S staining, ALP activit

SUBMITTER: Sun W 

PROVIDER: S-EPMC8411547 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature