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Development and validation of assays for the quantification of β-D-N⁴-hydroxycytidine in human plasma and β-D-N⁴-hydroxycytidine-triphosphate in peripheral blood mononuclear cell lysates.

ABSTRACT: The novel antiviral prodrug molnupiravir is under evaluation for the treatment of SARS-CoV-2. Molnupiravir is converted to β-D-N⁴-hydroxycytidine (NHC), which is the primary form found in systemic circulation. β-D-N⁴-hydroxycytidine-triphosphate (NHCtp) is the bioactive anabolite produced intracellularly. Sensitive and accurate bioanalytical methods are required to characterize NHC and NHCtp pharmacokinetics in clinical trials. Human K₂EDTA plasma or peripheral blood mononuclear cell (PBMC) lysates were spiked with NHC (plasma) or NHCtp (PBMC), respectively. Following the addition of isotopically-labeled internal standards and sample extraction via protein precipitation or lysate dilution, respectively, samples were subjected to liquid chromatographic-tande

SUBMITTER: Parsons TL

PROVIDER: S-EPMC8411588 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Dataset Information

Development and validation of assays for the quantification of β-D-N⁴-hydroxycytidine in human plasma and β-D-N⁴-hydroxycytidine-triphosphate in peripheral blood mononuclear cell lysates.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Development and validation of assays for the quantification of β-D-N4-hydroxycytidine in human plasma and β-D-N4-hydroxycytidine-triphosphate in peripheral blood mononuclear cell lysates.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Development and validation of assays for the quantification of β-D-N⁴-hydroxycytidine in human plasma and β-D-N⁴-hydroxycytidine-triphosphate in peripheral blood mononuclear cell lysates.