Clean and folded: Production of active, high quality recombinant human interferon-λ1.

ABSTRACT: Type III interferons exhibit antiviral activity against influenza viruses, coronaviruses, rotaviruses, and others. In addition, this type of interferon theoretically has therapeutic advantages, in comparison with type I interferons, due to its ability to activate a narrower group of genes in a relatively small group of target cells. Hence, it can elicit more targeted antiviral or immunomodulatory responses. Obtaining biologically-active interferon lambda (hIFN-λ₁) is fraught with difficulties at the stage of expression in soluble form or, in the case of expression in the form of inclusion bodies, at the stage of refolding. In this work, hIFN-λ₁ was expressed in the form of inclusion bodies, and a simple, effective refolding method was developed. Efficient and scalable

SUBMITTER: Shaldzhyan A 

PROVIDER: S-EPMC8411590 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature