Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline.

ABSTRACT: In situ microplates are small in size, crystal cultivation and operation are difficult, and the efficiency of crystal screening is relatively low. To solve this problem, a novel combined crystallization plate was designed for high-throughput crystal cultivation and in situ data collection. A frame was used to hold 48 in situ microplates, and the in situ microplates were sealed on one side with an ultralow background-scattering Kapton film. An automatic liquid handler (Mosquito) was used to add a liquid drop to the in situ microplates in the frame, and CrystalClear HD tape was used to seal the frame. A sealed frame holding 48 microplates was developed as a novel combined crystallization plate and was used for crystal cultivation under different conditions and in situ data collection at the

SUBMITTER: Liang M 

PROVIDER: S-EPMC8411933 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature