Ontology highlight
ABSTRACT:
SUBMITTER: Pintilie G
PROVIDER: S-EPMC8411978 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Acta crystallographica. Section D, Structural biology 20210831 Pt 9
The process of turning 2D micrographs into 3D atomic models of the imaged macromolecules has been under rapid development and scrutiny in the field of cryo-EM. Here, some important methods for validation at several stages in this process are described. Firstly, how Fourier shell correlation of two independent maps and phase randomization beyond a certain frequency address the assessment of map resolution is reviewed. Techniques for local resolution estimation and map sharpening are also touched ...[more]