Computational Methods for Single-Particle Electron Cryomicroscopy.

ABSTRACT: Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for elucidating the three-dimensional structure of proteins and other biologically significant complexes at near-atomic resolution. It is an imaging method that does not require crystallization and can capture molecules in their native states. In single-particle cryo-EM, the three-dimensional molecular structure needs to be determined from many noisy two-dimensional tomographic projections of individual molecules, whose orientations and positions are unknown. The high level of noise and the unknown pose parameters are two key elements that make reconstruction a challenging computational problem. Even more challenging is the inference of structural variability and flexible motions when the individual mole

SUBMITTER: Singer A 

PROVIDER: S-EPMC8412055 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature