Analysis of protrusion dynamics in amoeboid cell motility by means of regularized contour flows.

ABSTRACT: Amoeboid cell motility is essential for a wide range of biological processes including wound healing, embryonic morphogenesis, and cancer metastasis. It relies on complex dynamical patterns of cell shape changes that pose long-standing challenges to mathematical modeling and raise a need for automated and reproducible approaches to extract quantitative morphological features from image sequences. Here, we introduce a theoretical framework and a computational method for obtaining smooth representations of the spatiotemporal contour dynamics from stacks of segmented microscopy images. Based on a Gaussian process regression we propose a one-parameter family of regularized contour flows that allows us to continuously track reference points (virtual markers) between successive cell contours. We

SUBMITTER: Schindler D 

PROVIDER: S-EPMC8412247 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature