The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.

ABSTRACT: RNA regulation is essential to successful reproduction. Messenger RNAs delivered from parent to progeny govern early embryonic development. RNA-binding proteins (RBPs) are the key effectors of this process, regulating the translation and stability of parental transcripts to control cell fate specification events prior to zygotic gene activation. The KH-domain RBP MEX-3 is conserved from nematode to human. It was first discovered in Caenorhabditis elegans, where it is essential for anterior cell fate and embryo viability. Here, we show that loss of the endogenous mex-3 3´UTR disrupts its germline expression pattern. An allelic series of 3´UTR deletion variants identify repressing regions of the UTR and demonstrate that repression is not precisely coupled to reproductive success. We also sho

SUBMITTER: Albarqi MMY 

PROVIDER: S-EPMC8412283 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature