Towards Secretome Standardization: Identifying Key Ingredients of MSC-Derived Therapeutic Cocktail.

ABSTRACT: The therapeutic potential of the conditioned medium (CM) derived from MSCs (mesenchymal stem/stromal cells) in disparate medical fields, from immunology to orthopedics, has been widely suggested by in vitro and in vivo evidences. Prior to MSC-CM use in clinical applications, appropriate quality controls are needed in order to assess its reproducibility. Here, we evaluated different CM characteristics, including general features and precise protein and lipid concentrations, in 3 representative samples from adipose-derived MSCs (ASCs). In details, we first investigated the size and distribution of the contained extracellular vesicles (EVs), lipid bilayer-delimited particles whose pivotal role in intercellular communication has been extensively demonstrated. Then, we acquired Ra

SUBMITTER: Giannasi C 

PROVIDER: S-EPMC8413055 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature