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Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni²⁺ magnetic beads.

ABSTRACT: To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni²⁺ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.

SUBMITTER: Conzentino MS

PROVIDER: S-EPMC8413102 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads.

Conzentino Marcelo S MS Santos Tatielle P C TPC Selim Khaled A KA Wagner Berenike B Alford Janette T JT Deobald Nelli N Paula Nigela M NM Rego Fabiane G M FGM Zanette Dalila L DL Aoki Mateus N MN Nardin Jeanine M JM Huergo Maria C C MCC Reis Rodrigo A RA Huergo Luciano F LF

Analytical biochemistry 20210903

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A sim ...[more]

PMID: 34481802

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