Chronic restraint stress induces changes in the cerebral Galpha 12/13 and Rho-GTPase signaling network.

ABSTRACT:

Background

Evidence indicates that Gα12, Gα13, and its downstream effectors, RhoA and Rac1, regulate neuronal morphology affected by stress. This study was aimed at investigating whether repeated stress influences the expression of proteins related to the Gα12/13 intracellular signaling pathway in selected brain regions sensitive to the effects of stress. Furthermore, the therapeutic impact of β(1)adrenergic receptors (β1AR) blockade was assessed.

Methods

Restraint stress (RS) model in mice (2 h/14 days) was used to assess prolonged stress effects on the mRNA expression of Gα12, Gα13, RhoA, Rac1 in the prefrontal cortex (PFC), hippocampus (HIP) and amygdala (AMY). In a separate study, applying RS model in rats (3-4 h/1 day or 14 days), we evaluated stress effects on the expr

SUBMITTER: Rafa-Zablocka K 

PROVIDER: S-EPMC8413188 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature