Characterization of dopamine D₂ receptor coupling to G proteins in postmortem brain of subjects with schizophrenia.

ABSTRACT:

Background

Alterations of dopamine D₁ (D1R) and D₂ receptor (D2R) are proposed in schizophrenia but brain neuroimaging and postmortem studies have shown controversial results in relation to D1R and D2R density. Besides, scarce information on the functionality of brain D1R and D2R is available. The present study characterized G-protein activation by D1R and D2R agonists in postmortem human brain. Furthermore, D2R functional status was compared between schizophrenia and control subjects.

Methods

G-protein receptor coupling was assessed in control caudate nucleus and frontal cortex by [³⁵S]GTPγS-binding stimulation induced by increasing concentrations (10^-10-10^-3 M) of dopamine, and the selective dopaminergic agonists SKF38

SUBMITTER: Egusquiza I 

PROVIDER: S-EPMC8413194 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature