Generation of Targeted Knockout Mutants in Arabidopsis thaliana Using CRISPR/Cas9.
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ABSTRACT: The CRISPR/Cas9 system has emerged as a powerful tool for gene editing in plants and beyond. We have developed a plant vector system for targeted Cas9-dependent mutagenesis of genes in up to two different target sites in Arabidopsis thaliana. This protocol describes a simple 1-week cloning procedure for a single T-DNA vector containing the genes for Cas9 and sgRNAs, as well as the detection of induced mutations in planta. The procedure can likely be adapted for other transformable plant species.
SUBMITTER: Hahn F
PROVIDER: S-EPMC8413554 | biostudies-literature |
REPOSITORIES: biostudies-literature
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