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Oil Red O Staining for Lipid Content in Caenorhabditis elegans.

ABSTRACT: The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an optimized protocol for ORO staining of C. elegans and provide detailed instructions for quantifying the intensity of ORO signal in images acquired by light microscopy.

SUBMITTER: Wang FY

PROVIDER: S-EPMC8413582 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Oil Red O Staining for Lipid Content in Caenorhabditis elegans.

Wang Feng-Yung FY Ching Tsui-Ting TT

Bio-protocol 20210820 16

The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an optimized protocol for ORO staining of C. elegans and provide detailed instructions for quantifying the intensity of ORO signal in im ...[more]

PMID: 34541042

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