Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach.

ABSTRACT:

Background

The horn fly, Haematobia irritans irritans, causes significant production losses to the cattle industry. Horn fly control relies on insecticides; however, alternative control methods such as vaccines are needed due to the fly's capacity to quickly develop resistance to insecticides, and the pressure for eco-friendly options.

Methods

We used a reverse vaccinology approach comprising three vaccine prediction and 11 annotation tools to evaluate and rank 79,542 translated open reading frames (ORFs) from the horn fly's transcriptome, and selected 10 transcript ORFs as vaccine candidates for expression in Pichia pastoris. The expression of the 10 selected transcripts and the proteins that they encoded were investigated in adult flies by reverse transcription polymerase

SUBMITTER: Domingues LN 

PROVIDER: S-EPMC8414034 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature