Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm.

ABSTRACT:

Background

Knowledge on the localization and mobility of enzymes inside bacterial cells is scarce, but important for understanding spatial regulation of metabolism. The four central enzymes (Rib enzymes) of the riboflavin (RF) biosynthesis pathway in the Gram positive model bacterium Bacillus subtilis have been studied extensively in vitro, especially the heavy RF synthase, a large protein complex with a capsid structure formed by RibH and an encapsulated RibE homotrimer, which mediates substrate-channeling. However, little is known about the behavior and mobility of these enzymes in vivo.

Results

We have investigated the localization and diffusion of the Rib enzymes in the cytoplasm of B. subtilis. By characterizing the diffusion of Rib enzymes in live cells using single pa

SUBMITTER: Rotter DAO 

PROVIDER: S-EPMC8414670 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature