Single worm transcriptomics identifies a developmental core network of oscillating genes with deep conservation across nematodes.

ABSTRACT: High-resolution spatial and temporal maps of gene expression have facilitated a comprehensive understanding of animal development and evolution. In nematodes, the small body size represented a major challenge for such studies, but recent advancements have helped overcome this limitation. Here, we have implemented single worm transcriptomics (SWT) in the nematode model organism Pristionchus pacificus to provide a high-resolution map of the developmental transcriptome. We selected 38 time points from hatching of the J2 larvae to young adults to perform transcriptome analysis over 60 h of postembryonic development. A mean sequencing depth of 4.5 million read pairs allowed the detection of more than 23,135 (80%) of all genes. Nearly 3000 (10%) genes showed oscillatory expression with di

SUBMITTER: Sun S 

PROVIDER: S-EPMC8415380 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature