Contaminants and Where to Find Them: Microbiological Quality Control in Axenic Animal Facilities.

ABSTRACT: The use of axenic animal models in experimental research has exponentially grown in the past few years and the most reliable way for confirming their axenic status remains unclear. It is especially the case when using individual ventilated positive-pressure cages such as the Isocage. This type of cage are at a greater risk of contamination and expose animals to a longer handling process leading to more potential stress when opened compared to isolators. The aim of this study was to propose simple ways to detect microbial contaminants with Isocages type isolator resulting by developing, validating and optimizing three different methods (culture, microscopy, and molecular). These three approaches were also tested in situ by spiking 21 axenic mice with different microorganisms. Our res

SUBMITTER: Lebeuf M 

PROVIDER: S-EPMC8415547 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature