Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas.

ABSTRACT:

Purposes

Although clinical and radiological examinations can be used to diagnose oral cancer, and surgical pathology remains the gold standard, these conventional methods have limitations. We evaluated the feasibility of longitudinal next-generation sequencing-based liquid biopsy for oral squamous cell carcinoma surveillance.

Materials and methods

Eleven patients were enrolled, and plasma and saliva were collected before, and 1, 3, and 6 months after surgery. Tumor-specific mutations were selected using paired, whole-exome analyses of tumor tissues and whole blood. Genes frequently mutated in head and neck cancer were identified using the Cancer Genome Atlas (TCGA) and Catalogue of Somatic Mutations in Cancer (COSMIC) databases to design targeted deep sequencing panels.

R

SUBMITTER: Cui Y 

PROVIDER: S-EPMC8415592 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature