Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis.

ABSTRACT: Multiplex engineering at the scale of whole genomes has become increasingly important for synthetic biology and biotechnology applications. Although several methods have been reported for engineering microbe genomes, their use is limited by their complex procedures using multi-cycle transformations. Natural transformation, involving in species evolution by horizontal gene transfer in many organisms, indicates its potential as a genetic tool. Here, we aimed to develop simultaneous multiplex genome engineering (SMGE) for the simple, rapid, and efficient design of bacterial genomes via one-step of natural transformation in Bacillus subtilis. The transformed DNA, competency factors, and recombinases were adapted to improved co-editing frequencies above 27-fold. Single to octuplet

SUBMITTER: Deng A 

PROVIDER: S-EPMC8416114 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature