Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays.

ABSTRACT: Species identification within the genus Mycobacterium and subsequent antibiotic susceptibility testing still rely on time-consuming, culture-based methods. Despite the recent development of DNA probes, which greatly reduce assay time, there is a need for a single platform assay capable of answering the multitude of diagnostic questions associated with this genus. We describe the use of a DNA probe array based on two sequence databases: one for the species identification of mycobacteria (82 unique 16S rRNA sequences corresponding to 54 phenotypical species) and the other for detecting Mycobacterium tuberculosis rifampin resistance (rpoB alleles). Species identification or rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacte

SUBMITTER: Troesch A 

PROVIDER: S-EPMC84166 | biostudies-literature | 1999 Jan

REPOSITORIES: biostudies-literature