Dataset Information

Isoleucine Enhances Plant Resistance Against Botrytis cinerea via Jasmonate Signaling Pathway.

ABSTRACT: Amino acids are the building blocks of biomacromolecules in organisms, among which isoleucine (Ile) is the precursor of JA-Ile, an active molecule of phytohormone jasmonate (JA). JA is essential for diverse plant defense responses against biotic and abiotic stresses. Botrytis cinerea is a necrotrophic nutritional fungal pathogen that causes the second most severe plant fungal disease worldwide and infects more than 200 kinds of monocot and dicot plant species. In this study, we demonstrated that Ile application enhances plant resistance against B. cinerea in Arabidopsis, which is dependent on the JA receptor COI1 and the jasmonic acid-amido synthetase JAR1. The mutant lib with higher Ile content in leaves exhibits enhanced resistance to B. cinerea infection. Furthermore, we found that the exogenous Ile application moderately enhanced plant resistance to B. cinerea in various horticultural plant species, including lettuce, rose, and strawberry, suggesting a practical and effective strategy to control B. cinerea disease in agriculture. These results together showed that the increase of Ile could positively regulate the resistance of various plants to B. cinerea by enhancing JA signaling, which would offer potential applications for crop protection.

SUBMITTER: Li Y

PROVIDER: S-EPMC8416682 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Isoleucine Enhances Plant Resistance Against <i>Botrytis cinerea</i> via Jasmonate Signaling Pathway.

Li Yuwen Y Li Suhua S Du Ran R Wang Jiaojiao J Li Haiou H Xie Daoxin D Yan Jianbin J

Frontiers in plant science 20210819

Amino acids are the building blocks of biomacromolecules in organisms, among which isoleucine (Ile) is the precursor of JA-Ile, an active molecule of phytohormone jasmonate (JA). JA is essential for diverse plant defense responses against biotic and abiotic stresses. <i>Botrytis cinerea</i> is a necrotrophic nutritional fungal pathogen that causes the second most severe plant fungal disease worldwide and infects more than 200 kinds of monocot and dicot plant species. In this study, we demonstrat ...[more]

PMID: 34489985

Similar Datasets

Project description:Free and glycosylated sterols are both structural components of the plasma membrane that regulate their biophysical properties and consequently different plasma membrane-associated processes such as plant adaptation to stress or signaling. Several reports relate changes in glycosylated sterols levels with the plant response to abiotic stress, but the information about the role of these compounds in the response to biotic stress is scarce. In this work, we have studied the response to the necrotrophic fungus Botrytis cinerea in an Arabidopsis mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases (UGT80A2 and UGT80B1) reported in this plant. This mutant exhibits enhanced resistance against B. cinerea when compared to wild-type plants, which correlates with increased levels of jasmonic acid (JA) and up-regulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Upon B. cinerea infection, the ugt80A2;B1 double mutant also accumulates higher levels of camalexin, the major Arabidopsis phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also up-regulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants. Altogether, the results of this study show that glycosylated sterols play an important role in the regulation of Arabidopsis response to B. cinerea infection and suggest that this occurs through signaling pathways involving the canonical stress-hormone JA and the tryptophan-derived secondary metabolites camalexin and possibly also indole glucosinolates.

Dataset Information

Isoleucine Enhances Plant Resistance Against Botrytis cinerea via Jasmonate Signaling Pathway.

Publications

Isoleucine Enhances Plant Resistance Against <i>Botrytis cinerea</i> via Jasmonate Signaling Pathway.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets