Rapid Serial Immunoprofiling of the Tumor Immune Microenvironment by Fine Needle Sampling.

ABSTRACT:

Purpose

There is increasing effort to discover and integrate predictive and/or prognostic biomarkers into treatment algorithms. While tissue-based methods can reveal tumor-immune cell compositions at a single time point, we propose that single-cell sampling via fine needle aspiration (FNA) can facilitate serial assessment of the tumor immune microenvironment (TME) with a favorable risk-benefit profile.

Experimental design

Primary antibodies directed against 20 murine and 25 human markers of interest were chemically modified via a custom linker-bio-orthogonal quencher (FAST) probe. A FAST-FNA cyclic imaging and analysis pipeline were developed to derive quantitative response scores. Single cells were harvested via FNA and characterized phenotypically and functionally both in

SUBMITTER: Oh J 

PROVIDER: S-EPMC8416923 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature