Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM): a sibling oocyte study.

ABSTRACT:

Purpose

To determine if euploidy rates and embryo development differ when blastocysts are cultured in CCM or SCM.

Method

A single-center retrospective observational study was performed from September 2018 to March 2019. Patients [23-46 years] with at least four fresh mature oocytes (MII) without severe male factor infertility were included. Sibling MII were injected and cultured in Global®Total®LP (CCM) or Sage Quinn's Advantage® Cleavage and Blastocyst media (SCM) under 6% CO₂, 5% O₂, and 89% N₂. Fertilization, cleavage, day (D) 5 blastulation, usable blastocyst (blastocysts biopsied/normally fertilized oocytes), and euploidy rates were recorded. Blastocysts were graded prior to trophectoderm (TE) biopsy on D5, 6, or 7 for genetic testing an

SUBMITTER: Abdala A 

PROVIDER: S-EPMC8417167 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature