Sperm DNA integrity status is associated with DNA methylation signatures of imprinted genes and non-imprinted genes.

ABSTRACT:

Purpose

To evaluate the association between the DNA methylation of specific genes and sperm DNA integrity status in human sperm samples.

Methods

A total of 166 semen samples were evaluated (86 controls and 80 cases with impaired sperm DNA integrity). We detected the methylation status of 257 CpG sites among two imprinted genes (H19 and SNRPN) and four non-imprinted genes related to male infertility (MTHFR, GSTM1, DAZL, and CREM) by using a targeted next-generation sequencing method.

Results

Differential methylation was found in 43 CpG sites of the promoters of the six candidate genes. H19, SNRPN, MTHFR, DAZL, GSTM1, and CREM contained 22, 12, 1, 4, 0, and 4 differentially methylated CpG sites (P<0.05), respectively. The imprinting genes were associated with relatively

SUBMITTER: Song B 

PROVIDER: S-EPMC8417181 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature