A cortex-specific penicillin-binding protein contributes to heat resistance in Clostridioides difficile spores.

ABSTRACT:

Background

Sporulation is a complex cell differentiation programme shared by many members of the Firmicutes, the end result of which is a highly resistant, metabolically inert spore that can survive harsh environmental insults. Clostridioides difficile spores are essential for transmission of disease and are also required for recurrent infection. However, the molecular basis of sporulation is poorly understood, despite parallels with the well-studied Bacillus subtilis system. The spore envelope consists of multiple protective layers, one of which is a specialised layer of peptidoglycan, called the cortex, that is essential for the resistant properties of the spore. We set out to identify the enzymes required for synthesis of cortex peptidoglycan in C. difficile.

Methods

Bioi

SUBMITTER: Alabdali YAJ 

PROVIDER: S-EPMC8417463 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature