Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development.

ABSTRACT:

Background

A number of cellular processes have evolved in metazoans that increase the proteome repertoire in relation to the genome, such as alternative splicing and translation recoding. Another such process, translational stop codon readthrough (SCR), generates C-terminally extended protein isoforms in many eukaryotes, including yeast, plants, insects, and humans. While comparative genome analyses have predicted the existence of programmed SCR in many species including humans, experimental proof of its functional consequences are scarce.

Results

We show that SCR of the Drosophila POU/Oct transcription factor Ventral veins lacking/Drifter (Vvl/Dfr) mRNA is prevalent in certain tissues in vivo, reaching a rate of 50% in the larval prothoracic gland. Phylogenetically, the C-t

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8417969 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature