Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides.

ABSTRACT:

Background

Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear.

Methods

The total proteins in pollen of P. deltoides were analyzed by proteomics, and the potential allergens were identified via the WHO/IUIS database and the allergenOnline database retrieval. One target protein was screened by bioinformatics and expressed in Escherichia coli. The biological activity of the expressed product was verified by animal experiments.

Results

The total of 3929 proteins in pollen of P. deltoides were identified, and 46 potential allergens belonging to 10 protein families were recognized by database retrieval. B9N9W6 protein of Hsp70 family was screened by bioinformatics analysis and expressed successfully. ELISA showed that

SUBMITTER: Guo W 

PROVIDER: S-EPMC8417992 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature