Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica).

ABSTRACT:

Background

Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in plants, but this ability is essential for most genetic research and molecular breeding.

Results

We developed a system for genotyping by quantitative PCR (qPCR) that allowed flesh color genotyping in multiple tetraploid and triploid loquat varieties (lines). The analysis of 13 different ratios of DNA mixtures between two homozygous diploids (AA and aa) showed that the proportion of allele A has a high correlation (R² = 0.9992) with parameter b [b = a₁/(a₁ + a₂)], which is derived from the two normalized allele signals (

SUBMITTER: Wang H 

PROVIDER: S-EPMC8418031 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature