Unknown

Dataset Information

0

Whole Genome Sequencing of Pediatric Klebsiella pneumoniae Strains Reveals Important Insights Into Their Virulence-Associated Traits.


ABSTRACT: Klebsiella pneumoniae is recognized as a common cause of nosocomial infections and outbreaks causing pneumonia, septicemia, and urinary tract infections. This opportunistic bacterium shows an increasing acquisition of antibiotic-resistance genes, which complicates treatment of infections. Hence, fast reliable strain typing methods are paramount for the study of this opportunistic pathogen's multi-drug resistance genetic profiles. In this study, thirty-eight strains of K. pneumoniae isolated from the blood of pediatric patients were characterized by whole-genome sequencing and genomic clustering methods. Genes encoding β-lactamase were found in all the bacterial isolates, among which the bla SHV variant was the most prevalent (53%). Moreover, genes encoding virulence factors such as fimbriae, capsule, outer membrane proteins, T4SS and siderophores were investigated. Additionally, a multi-locus sequence typing (MLST) analysis revealed 24 distinct sequence types identified within the isolates, among which the most frequently represented were ST76 (16%) and ST70 (11%). Based on LPS structure, serotypes O1 and O3 were the most prevalent, accounting for approximately 63% of all infections. The virulence capsular types K10, K136, and K2 were present in 16, 13, and 8% of the isolates, respectively. Phylogenomic analysis based on virtual genome fingerprints correlated with the MLST data. The phylogenomic reconstruction also denoted association between strains with a higher abundance of virulence genes and virulent serotypes compared to strains that do not possess these traits. This study highlights the value of whole-genomic sequencing in the surveillance of virulence attributes among clinical K. pneumoniae strains.

SUBMITTER: Flores-Valdez M 

PROVIDER: S-EPMC8418058 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6562249 | biostudies-literature
| S-EPMC4325807 | biostudies-literature
| S-EPMC5541162 | biostudies-literature
| S-EPMC7400116 | biostudies-literature
| S-EPMC4432395 | biostudies-literature
| S-EPMC5471223 | biostudies-literature
| S-EPMC6408229 | biostudies-literature
| S-EPMC6102385 | biostudies-literature
| S-EPMC6414388 | biostudies-literature
| S-EPMC7277725 | biostudies-literature