Polycistronic Expression System for Pichia pastoris Composed of Chitino- and Chitosanolytic Enzymes.

ABSTRACT: Chitin is one of the most abundant biopolymers. Due to its recalcitrant nature and insolubility in accessible solvents, it is often considered waste and not a bioresource. The products of chitin modification such as chitosan and chitooligosaccharides are highly sought, but their preparation is a challenging process, typically performed with thermochemical methods that lack specificities and generate hazardous waste. Enzymatic treatment is a promising alternative to these methods, but the preparation of multiple biocatalysts is costly. In this manuscript, we biochemically characterised chitin deacetylases of Mucor circinelloides IBT-83 and utilised one of them for the construction of the first eukaryotic, polycistronic expression system employing self-processing 2A sequences. The thr

SUBMITTER: Kaczmarek MB 

PROVIDER: S-EPMC8418187 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature