A new method for anti-negative interference of calcium dobesilate in serum creatinine enzymatic analysis.

ABSTRACT:

Background

Serum creatinine is a widely used biomarker for evaluating renal function. Sarcosine oxidase enzymatic (SOE) analysis is currently the most widely used method for the detection of creatinine. This method was negatively interfered with by calcium dobesilate, causing pseudo-reduced results. The aim of this study was to explore a new method to alleviate the negative interference of this drug on creatinine detection.

Method

We formulated eight drug concentrations and 12 creatinine concentrations from serum. The SOE method, the new method, and the Jaffe method were used for detection in five systems. Creatinine biases were analyzed under the conditions with or without the interference of calcium dobesilate, at consistent or inconsistent creatinine concentrations. Creat

SUBMITTER: Shen H 

PROVIDER: S-EPMC8418471 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature