Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types.

ABSTRACT:

Background

Liquid biopsy is gaining increasing popularity in cancer screening and diagnosis. However, there is no relatively mature DNA isolation method or commercial kit available that is compatible with different LB sample types. This study developed a PAN-sample DNA isolation method (PAN method) for liquid biopsy samples.

Methods

The PAN method has two key steps, including biosample-specific pretreatments for various LB sample types and high concentration guanidine thiocyanate buffer for lysis and denaturation procedure. Subsequently, the performance of PAN method was validated by a series of molecular analyses.

Results

The PAN method was used to isolate DNA from multiple sample types related to LB, including plasma, serum, saliva, nasopharyngeal swab, and stool. A

SUBMITTER: Chen XX 

PROVIDER: S-EPMC8418477 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature