ZFTA-RELA Dictates Oncogenic Transcriptional Programs to Drive Aggressive Supratentorial Ependymoma.

ABSTRACT: More than 60% of supratentorial ependymomas harbor a ZFTA-RELA (ZR^fus) gene fusion (formerly C11orf95-RELA). To study the biology of ZR^fus, we developed an autochthonous mouse tumor model using in utero electroporation (IUE) of the embryonic mouse brain. Integrative epigenomic and transcriptomic mapping was performed on IUE-driven ZR^fus tumors by CUT&RUN, chromatin immunoprecipitation sequencing, assay for transposase-accessible chromatin sequencing, and RNA sequencing and compared with human ZR^fus-driven ependymoma. In addition to direct canonical NFκB pathway activation, ZR^fus dictates a neoplastic transcriptional program and binds to thousands of unique sites across the genome that are enriched with PLAGL family

SUBMITTER: Arabzade A 

PROVIDER: S-EPMC8418998 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature