Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids.

ABSTRACT: How stem cells self-organize to form structured tissues is an unsolved problem. Intestinal organoids offer a model of self-organization as they generate stem cell zones (SCZs) of typical size even without a spatially structured environment. Here we examine processes governing the size of SCZs. We improve the viability and homogeneity of intestinal organoid cultures to enable long-term time-lapse imaging of multiple organoids in parallel. We find that SCZs are shaped by fission events under strong control of ion channel-mediated inflation and mechanosensitive Piezo-family channels. Fission occurs through stereotyped modes of dynamic behavior that differ in their coordination of budding and differentiation. Imaging and single-cell transcriptomics show that inflation drives acute stem cell di

SUBMITTER: Tallapragada NP 

PROVIDER: S-EPMC8419000 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature