P53 Frameshift Mutations Couple Loss-of-Function with Unique Neomorphic Activities.

ABSTRACT: p53 mutations that result in loss of transcriptional activity are commonly found in numerous types of cancer. While the majority of these are missense mutations that map within the central DNA-binding domain, truncations and/or frameshift mutations can also occur due to various nucleotide substitutions, insertions, or deletions. These changes result in mRNAs containing premature stop codons that are translated into a diverse group of C-terminally truncated proteins. Here we characterized three p53 frameshift mutant proteins expressed from the endogenous TP53 locus in U2OS osteosarcoma and HCT116 colorectal cancer cell lines. These mutants retain intact DNA-binding domains but display altered oligomerization properties. Despite their abnormally high expression levels, they are mostly

SUBMITTER: Tong DR 

PROVIDER: S-EPMC8419077 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature