A sick plot-based protocol for dry root rot disease assessment in field-grown chickpea plants.

ABSTRACT:

Premise

A comprehensive field-based screening protocol is lacking for dry root rot (DRR) disease in chickpea, which is caused by Macrophomina phaseolina (formerly referred to as Rhizoctonia bataticola). Here, we describe a protocol for establishing a sick plot for DRR to enable disease assessment of a large number of chickpea plants during the natural growing season.

Methods and results

We used a chickpea plot with >30% DRR incidence, and enriched the inoculum by cultivating highly susceptible chickpea plant genotypes and incorporating infected plant material into the soil. The chickpea plants were then subjected to infection in developed sick plots with various levels of soil moisture under natural field conditions.

Conclusions

Our protocol provides a r

SUBMITTER: Irulappan V 

PROVIDER: S-EPMC8419395 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature