RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex.

ABSTRACT: During neocortical development, neural stem cells (NSCs) divide symmetrically to self-renew at the early stage and then divide asymmetrically to generate post-mitotic neurons. The molecular mechanisms regulating the balance between NSC self-renewal and neurogenesis are not fully understood. Using mouse in utero electroporation (IUE) technique and in vitro human NSC differentiation models including cerebral organoids (hCOs), we show here that regulator of cell cycle (RGCC) modulates NSC self-renewal and neuronal differentiation by affecting cell cycle regulation and spindle orientation. RGCC deficiency hampers normal cell cycle process and dysregulates the mitotic spindle, thus driving more cells to divide asymmetrically. These modulations diminish the NSC population and cause NSC pre-diffe

SUBMITTER: Guo Z 

PROVIDER: S-EPMC8419700 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature