Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection.
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ABSTRACT:
SUBMITTER: Thirunavukarasu D
PROVIDER: S-EPMC8419911 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection.
Thirunavukarasu Deepak D Cheng Lauren Y LY Song Ping P Chen Sherry X SX Borad Mitesh J MJ Kwong Lawrence L James Phillip P Turner Daniel J DJ Zhang David Yu DY
Genome biology 20210906 1
We develop the Oncogene Concatenated Enriched Amplicon Nanopore Sequencing (OCEANS) method, in which variants with low variant allele frequency (VAFs) are amplified and subsequently concatenated for Nanopore Sequencing. OCEANS allows accurate detection of somatic mutations with VAF limits of detection between 0.05 and 1%. We construct 4 distinct multi-gene OCEANS panels targeting recurrent mutations in acute myeloid leukemia, melanoma, non-small- cell lung cancer, and hepatocellular carcinoma an ...[more]
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