Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.

ABSTRACT:

Background

The identification of open chromatin regions and transcription factor binding sites (TFBs) is an important step in understanding the regulation of gene expression in diverse species. ATAC-seq is a technique used for such purpose by providing high-resolution measurements of chromatin accessibility revealed through integration of Tn5 transposase. However, the existence of cell walls in filamentous fungi and associated difficulty in purifying nuclei have precluded the routine application of this technique, leading to a lack of experimentally determined and computationally inferred data on the identity of genome-wide cis-regulatory elements (CREs) and TFBs. In this study, we constructed an ATAC-seq platform suitable for filamentous fungi and generated ATAC-seq libraries of A

SUBMITTER: Huang L 

PROVIDER: S-EPMC8419926 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature