The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis is one of the most important microorganisms for recombinant protein production. It possesses the GRAS (generally recognized as safe) status and a potent protein secretion capacity. Secretory protein production greatly facilitates downstream processing and thus significantly reduces costs. However, not all heterologous proteins are secreted and intracellular production poses difficulties for quantification. To tackle this problem, we have established a so-called intracellular split GFP (iSplit GFP) assay in B. subtilis as a tool for the in vivo protein detection during expression in batch cultures and at a single-cell level. For the iSplit GFP assay, the eleventh β-sheet of sfGFP is fused to a target protein and can complement a detector protein consisti

SUBMITTER: Lenz P 

PROVIDER: S-EPMC8419962 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature